Functional characterization of heat shock protein 90 targeted compounds.

TitleFunctional characterization of heat shock protein 90 targeted compounds.
Publication TypeJournal Article
Year of Publication2013
AuthorsCruz IN, Zhang Y, de la Fuente M, Schätzlein A, Yang M
JournalAnal Biochem
Date Published2013 Jul 15
KeywordsAdenosine Triphosphatases, Antineoplastic Agents, Biological Assay, HSP90 Heat-Shock Proteins, Inhibitory Concentration 50, Protein Binding

Heat shock protein 90 (Hsp90) is an attractive cancer target that possesses two potential binding domains at the C and N termini. Nevertheless, assays that can reliably distinguish between the C- and N-terminal Hsp90 inhibitors and quantitatively characterize candidate drugs are limited. Here we report an Hsp90 binding assay and ATPase inhibition assay that can not only separate the C- and N-terminal inhibitors but also quantitatively determine their respective IC50 values.

Alternate JournalAnal. Biochem.
PubMed ID23563445